LOCUS       pLV-[hsa-miR-26a-1]        9131 bp    DNA     circular     15-MAR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7052..7912
                     /label=Amp(R)
     promoter        8113..8653
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5657..5891
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6466..6921)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5963..6093
                     /label=SV40 pA
     misc_feature    6094..6307
                     /label=SV40 ori
     misc_feature    4978..5574
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3596
                     /label=intron
     CDS             3643..4968
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3386
                     /label=hsa-mir-26a-1 precursor
BASE COUNT     2161 a      2387 c      2489 g      2094 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg tgatatcaca 
     3121 aggtcccagg gctggggtca gaaattctct cccgagggaa tgaagccaca ggagccaaga 
     3181 gcaggaggac caaggccctg gcgaaggccg tggcctcgtt caagtaatcc aggataggct 
     3241 gtgcaggtcc caatgggcct attcttggtt acttgcacgg ggacgcgggc ctggacgccg 
     3301 gcatccgggc tcaggacccc cctctctgcc agaggcacca acaccagagt tcacaaatca 
     3361 gtctcctgcc ctttgcatgt agcaaagcta gctcgagctt ttggagtacg tcgtctttag 
     3421 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag 
     3481 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat 
     3541 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt 
     3601 cgtgaaaact acccctctag agtcgagcta ccggtcgcca ccatggtgag caagggcgag 
     3661 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc 
     3721 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc 
     3781 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg 
     3841 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac 
     3901 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 
     3961 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag 
     4021 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg 
     4081 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc 
     4141 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac 
     4201 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc 
     4261 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac 
     4321 tccaccggcg gcatggacga gctgtacaag gacccaccgg tcgccaccat gaccgagtac 
     4381 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 
     4441 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 
     4501 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 
     4561 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 
     4621 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 
     4681 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 
     4741 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 
     4801 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 
     4861 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc 
     4921 ccgaaggacc gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgagc ggccgcgtcg 
     4981 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 
     5041 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 
     5101 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 
     5161 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 
     5221 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 
     5281 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 
     5341 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 
     5401 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 
     5461 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 
     5521 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc 
     5581 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 
     5641 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt 
     5701 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 
     5761 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 
     5821 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 
     5881 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 
     5941 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 
     6001 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     6061 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg 
     6121 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 
     6181 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 
     6241 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg 
     6301 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 
     6361 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 
     6421 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg 
     6481 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 
     6541 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 
     6601 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 
     6661 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 
     6721 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 
     6781 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 
     6841 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 
     6901 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat 
     6961 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 
     7021 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 
     7081 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 
     7141 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 
     7201 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 
     7261 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 
     7321 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 
     7381 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 
     7441 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 
     7501 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 
     7561 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 
     7621 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 
     7681 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 
     7741 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 
     7801 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 
     7861 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 
     7921 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 
     7981 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 
     8041 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 
     8101 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 
     8161 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 
     8221 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 
     8281 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 
     8341 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 
     8401 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 
     8461 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 
     8521 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 
     8581 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 
     8641 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 
     8701 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 
     8761 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 
     8821 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 
     8881 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 
     8941 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 
     9001 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 
     9061 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 
     9121 gctggagctg c 
//
